Effect of Calcium Channel Antagonist on Testicular Ischemia- Reperfusion Injury: An Experimental Study

Effect of Calcium Channel Antagonist on Testicular Ischemia- Reperfusion Injury: An Experimental Study
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Title:Effect of Calcium Channel Antagonist on Testicular Ischemia- Reperfusion Injury: An Experimental Study
Author/Abstract:Je Sean S. Gocson, MD; Darwin L. Lim, MD; Joseph R. Cortero, MD; Enrique Ian S. Lorenzo, MD and Edgardo L. Reyes, MD

Department of Urology, Jose R. Reyes Memorial Medical Center


Objective: To determine the effect of calcium channel antagonist in reducing testicular damage after ischemia-reperfusion (I-R) injury in rabbits. Materials and Methods: Three groups of male rabbits were included in the study: 1) Baseline group (n = 4), underwent bilateral orchiectomy 2) Control group (n = 18), testicular torsion simulated for 4 hours; 3) Calcium channel antagonist (CCA) group (n = 18), testicular torsion simulated for 4 hours and calcium channel antagonist administered during the last 30 minutes of ischemia. The latter 2 groups underwent bilateral orchiectomy after one week. The architecture and germinal epithelial cell thickness were determined by histological examination on each testis. The average thickness (in cell layers) of the calcium channel antagonist group was compared with the control group using the T-test. Results: The CCA group showed 10 percent more preserved thickness of germinal cell layer than the control group. However, statistical analysis revealed that they are not significant. Conclusion: CCA therapy administered during testicular ischemia-reperfusion injury resulted in a trend towards an increase in preserved germinal cell layer and reduced cell damage. However, the increase in the preserved thickness of germinal cell layers were not significant compared to the control group.

Key words: testicular torsion, ischemia-reperfusion injury, reactive oxygen species, calcium channel antagonist.
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