A Major Step Toward Realizing Renal Regeneration

FG_AUTHORS: Medical News Today

Main Category: Urology / Nephrology
Also Included In: Stem Cell Research
Article Date: 29 Jan 2013 - 0:00 PST

Current ratings for:
A Major Step Toward Realizing Renal Regeneration


Healthcare Prof:A Major Step Toward Realizing Renal Regeneration

The research group led by Associate Professor Kenji Osafune and his colleague Shin-ichi Mae, both from Center for iPS Cell Research and Application (CiRA), Kyoto University in Japan, has succeeded in developing a highly efficient method of inducing human induced pluripotent stem (iPS) cells to differentiate into intermediate mesoderm, the precursor of kidney, gonad, and other cell lineages. This represents a major step toward realizing renal regeneration.

As nearly all kidney cells are derived through differentiation from intermediate mesoderm, to realize kidney regeneration requires first the development of an efficient technology for differentiating human iPS or embryonic stem (ES) cells into intermediate mesoderm.

The research team established a method through which fluorescent protein can be readily inserted into the human iPS/ES cell genome through homologous recombination and used it in human iPS cells to successfully introduce green fluorescent protein (GFP) into Odd-skipped related 1: (OSR1), a marker gene for intermediate mesoderm differentiation. This makes it possible to ascertain whether differentiation into the target intermediate mesoderm cells has been achieved.

The system was then used to establish a protocol for inducing iPS cell differentiation into intermediate mesoderm which produced a high success rate of 90% or more. It was confirmed that the resulting human intermediate mesoderm was able to differentiate into various types of kidney cell, and renal tubule structures were successfully generated.

The findings indicate the possibility of using iPS cells to create a supply of cells for use in renal regenerative medicine. The differentiation system developed by the researchers is also expected to provide a new research tool to help elucidate the developmental mechanism of intermediate mesoderm.

The next step required is to develop a technique that allows efficient and specific differentiation into kidney cells using intermediate mesoderm derived from human iPS/ES cells. As intermediate mesoderm is known to differentiate into the three different lineages of kidney, adrenal cortex, and gonad cells, the new technique has potential application in regenerative medicine not only for the kidney but also for the adrenal cortex and gonad.

Article adapted by Medical News Today from original press release. Click 'references' tab above for source.
Visit our urology / nephrology section for the latest news on this subject.
Please use one of the following formats to cite this article in your essay, paper or report:

MLA

n.p. "A Major Step Toward Realizing Renal Regeneration." Medical News Today. MediLexicon, Intl., 29 Jan. 2013. Web.
29 Jan. 2013. <http://www.medicalnewstoday.com/releases/255467.php>


APA

Please note: If no author information is provided, the source is cited instead.



'A Major Step Toward Realizing Renal Regeneration'Please note that we publish your name, but we do not publish your email address. It is only used to let you know when your message is published. We do not use it for any other purpose. Please see our privacy policy for more information.

If you write about specific medications or operations, please do not name health care professionals by name.

All opinions are moderated before being included (to stop spam)

Contact Our News Editors

For any corrections of factual information, or to contact the editors please use our feedback form.

Please send any medical news or health news press releases to:

Note: Any medical information published on this website is not intended as a substitute for informed medical advice and you should not take any action before consulting with a health care professional. For more information, please read our terms and conditions.

Read more http://www.medicalnewstoday.com/releases/255467.php

PUA Social Network

Twitter Facebook

Contact us

The Philippine Urological Association,Inc
3rd floor. PCS Bldg., 992 EDSA, Quezon City,
Metro Manila, PHILIPPINES 1105
View Map

+632-9256740 
+632-4544439
+63917-5244456

eMail us